prime script rtmaster mix kit (TaKaRa)

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TaKaRa prime script rtmaster mix kit
Prime Script Rtmaster Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rtmaster+mix+kit/PrimeScript+RT+Master+Mix/pm41238541-385-15-20
Average 99 stars, based on 26928 article reviews

prime script rtmaster mix kit - by Bioz Stars, 2026-10

99/100 stars

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Article Title: RIPK3-Mediated Necroptosis in Diabetic Cardiomyopathy Requires CaMKII Activation.
Article Snippet: The cDNA was then synthesized by the Prime ScriptTM RTMaster Mix Kit (Takara, Kyoto, Japan) before quantitative real-time PCRwere performed using the SYBR Green Fast qPCR mix (Takara) with the ABI StepOne PCR System (ABI, Carlsbad, CA, USA).

Isolation:

Article Title: A serine protease inhibitor camostat mesilate prevents podocyte apoptosis and attenuates podocyte injury in metabolic syndrome model rats.
Article Snippet: .. Total RNA was extracted with an SV Total RNA Isolation Kit (Promega, Madison, MI), and 500 ng of total RNA was reverse-transcribed into cDNA using a Prime Script RTMaster Mix kit (Takara Bio Inc, Shiga, Japan). .. TaqMan probes for GAPDH, adiponectin, monocyte chemoattractant protein-1 (MCP-1), plasminogen activator inhibitor-1 (PAI-1), TNF-a, IL-6, IL-1b, nephrin, and podocin were purchased from Applied Biosystems (Foster City, CA, USA).

Reverse Transcription:

RNA Extraction:

Article Title: GLCCI1 rs37973
Article Snippet: .. Total RNA was extracted using RNAiso Plus (Total RNA extraction reagent, TaKaRa, Shiga, Japan), and cDNA was generated from 500 ng RNA using a Prime Script RTMaster Mix kit (TaKaRa). .. Real-time PCR was performed following the manufacturer’s instructions using SYBR Premix Ex Taq (Takara) with an ABI Prism 7500 detection system.

Generated:

Synthesized:

Article Title: A preserved TGFβ cytostatic response through DLD-mediated metabolic modulation undermines anti-TGFβ therapy in gastric cancer.
Article Snippet: For prokaryotic expression, DLD-WT, DLD-K430R, and DLD-K430Q were cloned into pET28A or pGEX vectors from Genecreate Biotechnology (Wuhan, China), and ACAT1was cloned into the pET-SUMO vector from Genecreate Biotechnology. .. Total RNA was extracted using TRIzol reagent, and first-strand cDNA was subsequently synthesized with the Prime Script RTMaster Mix Kit (Takara). .. This cDNA then served as the template for quantitative realtime PCR (qPCR), which was performed using the GoTaq qPCRMaster Mix (Promega) according to the manufacturer’s protocol.

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